Allow URL access, disallow subdirectory access

I have a second website stored in the root of my main website. It’s a subdomain with its own URL

I want users to access the second website via a URL eg

but not via the first website eg

Can this be done via .htaccess? If so where do I put the .htaccess file: in the root of the main site or the root of the subdirectory/domain?

Thank you.

Source: .htaccess

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